COS-7 cell stained for SEPT9 (orange) and Lamp1 (cyan)
COS-7 cell stained for endogenous α-tubulin (BOP orange) SEPT7 (BOP blue) and F-actin (BOP purple)
COS-7 cell stained for endogenous α-tubulin (BOP orange) SEPT7 (BOP blue) and F-actin (BOP purple)
Toddler Neuron
MDA-MB-231 expressing SEPT9_i1 GFP, stained for endogenous F-actin and TKS5
Baby Neuron
in vitro TIRF reconstitution assay of polymerizing actin (green) with actin seeds (magenta) and taxol-stabilized microtubules (red). Imaging performed at OMX.
in vitro TIRF reconstitution assay of polymerizing actin (green) with actin seeds (magenta) and taxol-stabilized microtubules (red) in the presence of 100nM SEPT6/7. Imaging performed at OMX.
in vitro TIRF reconstitution assay of dynamic microtubules (red) and GMPCPP MT seeds (green). Imaging performed at OMX.
in vitro TIRF reconstitution assay of KIF5B (red) with taxol-stabilized microtubules (cyan). Imaging performed at OMX.
Hippocampal neurons DIV4 co-transfected with mCherry and GFP-Lamp1 showing lysosomal trafficking in the axon. Shallow angle TIRF time lapse.
Actin movie